Jordan Krebs


I am currently a MD/PhD candidate pursuing a career in pathology and epigenomics. In the future, I want to run a lab focused on the epigenomics of pre-stressed, stressed, and pathological states of tissues. I hope to use my work to diagnose novel disease subtypes and understand the molecular mechanisms of organ preconditioning, especially ischemic preconditioning of the heart. In addition, I want to help form the framework of clinical epigenomics by teaching at the medical and graduate levels.


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# Transcription
# Epigenetics
# Epigenomic subtyping

Early History

01

Learned about antibody production while interning at Lampire Biological Laboratories.

02

Studied microbial taxonomy, microbial extremophiles, and organic chemistry in the Newman lab (Lycoming College), Venkateswaran lab (Caltech’s NASA-JPL), and McDonald lab (Lycoming college), respectively.

03

Earned a prestigious NIH UGSP scholarship during college which landed him in the Casellas lab (NIAMS/NIH) studying genome-editing and mammalian genomics.

04

Started medical training in the MD/PhD program at Penn State Hershey and joined the Pugh lab (Penn State -> Cornell) to pursue a career in medicine and epigenetics

Research

01

Genome-wide map of nucleosomes and sub-nucleosomes in human cells with a novel nuclease-based chromatin fragmentation strategy

In each cell, 2 meters of DNA is wrapped into about a 10 μM diameter space called the nucleus. DNA is coiled around histone octamers, collectively called a nucleosome. The position of nucleosomes determines what genes are accessible to nuclear proteins. We have devised a novel method in which a nuclease chews accessible DNA around these structures to determine their location in higher-resolution and to greater completion than previously published. A map of nucleosomes and sub-nucleosome particles genome-wide will provide a chromatin landscape for then surveying the location of transcription factors and otherchromatin complexes via nuclease-ChIP-exo (below).

Publications

Peer Reviewed Publications
01
A Pliable Mediator Acts as a Functional Rather than an Architectural Bridge between Promoters and Enhancers

Khattabi LE, Zhao H, Kalchschmidt J, Young N, Jung S, Blerkom PV, Kieffer-Kwon P, Kieffer-Kwon K, Park S, Wang X, Krebs J, Tripathi S, Sakabe N, Sobreira DR, Haung S, Rao SSP, Pruett N, Chauss D, Sadler E, Lopez A, Nobrega MA, Aiden EL, Asturias FJ, Casellas R.

Cell. 178(5):1145-1158.

Non-Peer Reviewed Publications
2014
CRISPR Design Tool and Protocol. figshare.

Krebs, J (2014). http://dx.doi.org/10.6084/m9.figshare.1117899.

Conferences/Posters/Talks

Talks
2021
“High-resolution mapping of transcription factor binding sites in human tissue”.

Krebs, J.

CEGR Mega meeting. Virtual. March 3, 2021.

2020
“Nuclease-ChIP-exo”.

Krebs, E.

CEGR Mega meeting. Feb. 4, 2020.

2019
“Prepping chromatin from solid organs and blood”.

Krebs, J.

CEGR Mega meeting. April 16, 2019.

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Poster Presentations
2019
“Optimizing chromatin fragmentation for mammalian ChIP-exo”.

Krebs, J, Lai, WKM, Pugh F.

2019 PSU MCIBS and Pathobiology retreat. Boalsburg, PA. Aug. 20, 2019. 2019 CEGR retreat. Boalsburg, PA. Oct. 12, 2019.

2018
“Application of ChIP-exo in human health.”

Krebs, J, Lai, WKM, Pugh F.

2018 CEGR retreat. Boalsburg, PA. Oct. 20, 2018.

2016
“The Systematic Knock-Out of Mediator Complex Subunits”.

Krebs J, Kieffer-Kwon P, Tripathi S, Jung S, Kieffer-kwon KR, Khattabi L, Casellas R.

2016 NIAMS Poster Day. Bethesda, MD. April 29, 2016; 2016 Post-bac Poster Day. Bethesda, MD. April 20, 2016.

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Conferences
2019
Chromatin and Epigenetic Regulation of Transcription, 38th Summer Symposium in Molecular Biology.
PSU. July 30-Aug. 2, 2019"

Mech. of Eukaryotic Transcription. Cold Spring Harbor Labs. Aug. 27-31, 2019.

Cell. 178(5):1145-1158.

2011
Amgen Scholars U.S. Symposium, University of California. Los Angeles, CA.
2010
Moravian College Undergraduate Math Student Conference Moravian College, Bethlehem, PA.